Microarray and suppression subtractive hybridization analyses of gene expression in hybrid poplar (Populus alba × Populus tremula var. glandulosa) cell suspension cultures after exposure to NaCl.
Identifieur interne : 002990 ( Main/Exploration ); précédent : 002989; suivant : 002991Microarray and suppression subtractive hybridization analyses of gene expression in hybrid poplar (Populus alba × Populus tremula var. glandulosa) cell suspension cultures after exposure to NaCl.
Auteurs : Eun-Kyung Bae [Corée du Sud] ; Hyoshin Lee ; Jae-Soon Lee ; Eun-Woon Noh ; Young-Im Choi ; Byung-Hyun Lee ; Dong-Woog ChoiSource :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2012.
Descripteurs français
- KwdFr :
- Banque de gènes (MeSH), Chlorure de sodium (pharmacologie), Expression des gènes (effets des médicaments et des substances chimiques), Gènes de plante (MeSH), Hybridation d'acides nucléiques (méthodes), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Stress physiologique (génétique), Séquençage par oligonucléotides en batterie (méthodes), Techniques de culture cellulaire (MeSH), Tolérance au sel (génétique), Étiquettes de séquences exprimées (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Expression des gènes, Populus.
- génétique : Populus, Protéines végétales, Stress physiologique, Tolérance au sel.
- métabolisme : Populus, Protéines végétales.
- méthodes : Hybridation d'acides nucléiques, Séquençage par oligonucléotides en batterie.
- pharmacologie : Chlorure de sodium.
- Banque de gènes, Gènes de plante, Techniques de culture cellulaire, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- Cell Culture Techniques (MeSH), Expressed Sequence Tags (MeSH), Gene Expression (drug effects), Gene Library (MeSH), Genes, Plant (MeSH), Nucleic Acid Hybridization (methods), Oligonucleotide Array Sequence Analysis (methods), Plant Proteins (genetics), Plant Proteins (metabolism), Populus (drug effects), Populus (genetics), Populus (metabolism), Salt Tolerance (genetics), Sodium Chloride (pharmacology), Stress, Physiological (genetics).
- MESH :
- chemical , genetics : Plant Proteins.
- drug effects : Gene Expression, Populus.
- genetics : Populus, Salt Tolerance, Stress, Physiological.
- chemical , metabolism : Plant Proteins, Populus.
- methods : Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis.
- chemical , pharmacology : Sodium Chloride.
- Cell Culture Techniques, Expressed Sequence Tags, Gene Library, Genes, Plant.
Abstract
The gene expression profiles of hybrid poplar (Populus alba × Populus tremula var. glandulosa) cells in suspension culture after exposure to salinity (NaCl) induced stress were examined by constructing two suppression subtractive hybridization (SSH) libraries. cDNA from non-treated cells was used as a driver and cDNA samples from cell suspension cultures exposed to 150 mM NaCl for 2 or 10 h were used as testers. Randomly selected clones from each SSH library were sequenced and 727 high-quality expressed sequence tags (ESTs) were obtained and analyzed. Four novel ESTs were identified. Between the two libraries, 542 unique SSH clones were selected for placement on a cDNA microarray. In total, 18 differentially expressed genes were identified with 4 and 12 genes being significantly differentially expressed 2 and 10 h after the treatment, respectively. Genes related to metabolism and protein synthesis and several genes whose protein products are implicated in salt or other abiotic stress-related responses were expressed in the salt-stressed cells.
DOI: 10.1016/j.plaphy.2012.04.015
PubMed: 22813944
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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substances chimiques)</term><term>Gènes de plante (MeSH)</term><term>Hybridation d'acides nucléiques (méthodes)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Stress physiologique (génétique)</term><term>Séquençage par oligonucléotides en batterie (méthodes)</term><term>Techniques de culture cellulaire (MeSH)</term><term>Tolérance au sel (génétique)</term><term>Étiquettes de séquences exprimées (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Expression des 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cultures exposed to 150 mM NaCl for 2 or 10 h were used as testers. Randomly selected clones from each SSH library were sequenced and 727 high-quality expressed sequence tags (ESTs) were obtained and analyzed. Four novel ESTs were identified. Between the two libraries, 542 unique SSH clones were selected for placement on a cDNA microarray. In total, 18 differentially expressed genes were identified with 4 and 12 genes being significantly differentially expressed 2 and 10 h after the treatment, respectively. Genes related to metabolism and protein synthesis and several genes whose protein products are implicated in salt or other abiotic stress-related responses were expressed in the salt-stressed cells.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22813944</PMID><DateCompleted><Year>2013</Year><Month>01</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>58</Volume><PubDate><Year>2012</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>Microarray and suppression subtractive hybridization analyses of gene expression in hybrid poplar (Populus alba × Populus tremula var. glandulosa) cell suspension cultures after exposure to NaCl.</ArticleTitle><Pagination><MedlinePgn>151-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.plaphy.2012.04.015</ELocationID><Abstract><AbstractText>The gene expression profiles of hybrid poplar (Populus alba × Populus tremula var. glandulosa) cells in suspension culture after exposure to salinity (NaCl) induced stress were examined by constructing two suppression subtractive hybridization (SSH) libraries. cDNA from non-treated cells was used as a driver and cDNA samples from cell suspension cultures exposed to 150 mM NaCl for 2 or 10 h were used as testers. Randomly selected clones from each SSH library were sequenced and 727 high-quality expressed sequence tags (ESTs) were obtained and analyzed. Four novel ESTs were identified. Between the two libraries, 542 unique SSH clones were selected for placement on a cDNA microarray. In total, 18 differentially expressed genes were identified with 4 and 12 genes being significantly differentially expressed 2 and 10 h after the treatment, respectively. Genes related to metabolism and protein synthesis and several genes whose protein products are implicated in salt or other abiotic stress-related responses were expressed in the salt-stressed cells.</AbstractText><CopyrightInformation>Copyright © 2012 Elsevier Masson SAS. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bae</LastName><ForeName>Eun-Kyung</ForeName><Initials>EK</Initials><AffiliationInfo><Affiliation>Division of Forest Biotechnology, Korea Forest Research Institute, 39 Onjeong-ro, Suwon 441-847, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Hyoshin</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jae-Soon</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Noh</LastName><ForeName>Eun-Woon</ForeName><Initials>EW</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Young-Im</ForeName><Initials>YI</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Byung-Hyun</ForeName><Initials>BH</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Dong-Woog</ForeName><Initials>DW</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>05</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>451W47IQ8X</RegistryNumber><NameOfSubstance UI="D012965">Sodium Chloride</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018929" MajorTopicYN="N">Cell Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020224" MajorTopicYN="N">Expressed Sequence Tags</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="Y">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055049" MajorTopicYN="N">Salt Tolerance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012965" MajorTopicYN="N">Sodium Chloride</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>03</Month><Day>31</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>04</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>7</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>7</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>1</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22813944</ArticleId><ArticleId IdType="pii">S0981-9428(12)00097-6</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2012.04.015</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Choi, Dong Woog" sort="Choi, Dong Woog" uniqKey="Choi D" first="Dong-Woog" last="Choi">Dong-Woog Choi</name><name sortKey="Choi, Young Im" sort="Choi, Young Im" uniqKey="Choi Y" first="Young-Im" last="Choi">Young-Im Choi</name><name sortKey="Lee, Byung Hyun" sort="Lee, Byung Hyun" uniqKey="Lee B" first="Byung-Hyun" last="Lee">Byung-Hyun Lee</name><name sortKey="Lee, Hyoshin" sort="Lee, Hyoshin" uniqKey="Lee H" first="Hyoshin" last="Lee">Hyoshin Lee</name><name sortKey="Lee, Jae Soon" sort="Lee, Jae Soon" uniqKey="Lee J" first="Jae-Soon" last="Lee">Jae-Soon Lee</name><name sortKey="Noh, Eun Woon" sort="Noh, Eun Woon" uniqKey="Noh E" first="Eun-Woon" last="Noh">Eun-Woon Noh</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Bae, Eun Kyung" sort="Bae, Eun Kyung" uniqKey="Bae E" first="Eun-Kyung" last="Bae">Eun-Kyung Bae</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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